{"id":3612,"date":"2019-09-19T21:41:06","date_gmt":"2019-09-19T19:41:06","guid":{"rendered":"https:\/\/www.bbemg.uliege.be\/expression-des-genes-suite-a-lexposition-aux-cem-rapports-dactivites-et-publications\/"},"modified":"2026-03-13T14:27:59","modified_gmt":"2026-03-13T13:27:59","slug":"expression-des-genes-suite-a-lexposition-aux-cem-rapports-dactivites-et-publications","status":"publish","type":"page","link":"https:\/\/www.bbemg.uliege.be\/fr\/expression-des-genes-suite-a-lexposition-aux-cem-rapports-dactivites-et-publications\/","title":{"rendered":"Expression des g\u00e8nes suite \u00e0 l\u2019exposition aux CEM : Rapports d’activit\u00e9s et publications"},"content":{"rendered":"[vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb][heading]\n

Expression des g\u00e8nes suite \u00e0 l\u2019exposition aux CEM : Rapports d’activit\u00e9s et publications<\/h1>\n[\/heading][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb source=\u00a0\u00bb\u00a0\u00bb text=\u00a0\u00bb2013 – 2017″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb google_fonts=\u00a0\u00bbfont_family:Abril%20Fatface%3Aregular|font_style:400%20regular%3A400%3Anormal\u00a0\u00bb css_animation=\u00a0\u00bb\u00a0\u00bb link=\u00a0\u00bb\u00a0\u00bb el_id=\u00a0\u00bb\u00a0\u00bb el_class=\u00a0\u00bb\u00a0\u00bb css=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb][vc_custom_heading text=\u00a0\u00bbRapports d'activit\u00e9s\u00a0\u00bb font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_custom_heading text=\u00a0\u00bb2023-2024″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_column_text]Cette recherche implique une collaboration \u00e9troite entre les \u00e9quipes du BBEMG :
– ULB-LROT (M. Hinsenkamp, JF. Collard, TTH. Nguyen<\/strong>)
– Sciensano (S. Segers, B. Mertens, R. Anthonissen, E. De Clercq, M. Ledent<\/strong>)<\/p>\n

Ce travail est maintenant termin\u00e9. Il a \u00e9t\u00e9 r\u00e9alis\u00e9 dans le cadre de la th\u00e8se de doctorat de Ha Nguyen. Le doctorat intitul\u00e9 INVESTIGATION ON THE LONG TERM GENETIC EFFECTS OF EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELDS a \u00e9t\u00e9 soutenu avec succ\u00e8s \u00e0 l\u2019\u00e9t\u00e9 2023.<\/p>\n

Pour consulter le manuscrit complet et en savoir plus :\u00a0cliquez ici<\/a>[\/vc_column_text][vc_custom_heading text=\u00a0\u00bb2022-2023″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_column_text]Cette recherche implique une collaboration \u00e9troite entre les \u00e9quipes du BBEMG :
– ULB-LROT (M. Hinsenkamp, JF. Collard, TTH. Nguyen<\/strong>)
– Sciensano (S. Segers, B. Mertens, L. Verschaeve, A. Maes, R. Anthonissen, E. De Clercq, M. Ledent<\/strong>)
Avant de proc\u00e9der \u00e0 l’analyse de l’expression des g\u00e8nes, une revue approfondie de la litt\u00e9rature des \u00e9tudes portant sur les effets de l’exposition aux CM sur l’expression des g\u00e8nes a \u00e9t\u00e9 r\u00e9alis\u00e9e. L’objectif \u00e9tait d’identifier et de s\u00e9lectionner une liste de g\u00e8nes int\u00e9ressants pour l’analyse de l’expression g\u00e9n\u00e9tique dans les cellules TK6 et les \u00e9chantillons sanguins et buccaux des travailleurs.
Dans l’analyse de l’expression g\u00e9n\u00e9tique des cellules TK6, bien que nous ayons d\u00e9tect\u00e9 des modifications dans l’expression de plusieurs g\u00e8nes des cellules expos\u00e9es au CM par rapport aux cellules non expos\u00e9es, l’ampleur des effets \u00e9tait faible. Les \u00e9tudes d’expression g\u00e9nique sur les \u00e9chantillons sanguins et buccaux des travailleurs n’ont r\u00e9v\u00e9l\u00e9 aucun g\u00e8ne r\u00e9gul\u00e9 par l’exposition au MF.[\/vc_column_text][vc_custom_heading text=\u00a0\u00bb2021-2022″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_column_text]Cette recherche implique une collaboration \u00e9troite entre les \u00e9quipes du BBEMG :
– ULB-LROT (M. Hinsenkamp, JF. Collard, TTH. Nguyen<\/strong>)
– Sciensano (M. Ledent, S. Segers, B. Mertens, L. Verschaeve, A. Maes, R. Anthonissen, J. Van De Maele, E. De Clercq<\/strong>)
– ULi\u00e8ge-ACE (C. Geuzaine, V. Beauvois, M. Spirlet, J. Arban<\/strong>)
Afin d’\u00e9tudier les effets g\u00e9n\u00e9tiques de l’exposition aux FEB-MF et de mieux comprendre le m\u00e9canisme d’action de l’exposition aux MF, une r\u00e9action en cha\u00eene par polym\u00e9rase quantitative en temps r\u00e9el (RT-qPCR) a \u00e9t\u00e9 appliqu\u00e9e \u00e0 des \u00e9chantillons de sang et de muqueuse buccale d’employ\u00e9s expos\u00e9s professionnellement aux CM-EBF. Notre objectif est d’identifier les g\u00e8nes dont l’expression peut changer suite \u00e0 une exposition aux CM.
L’analyse de l’expression g\u00e9n\u00e9tique sur les \u00e9chantillons sanguins et buccaux des employ\u00e9s sugg\u00e8re que l’exposition professionnelle aux CM-EBF ne modifie pas l’expression des g\u00e8nes impliqu\u00e9s dans plusieurs processus cellulaires, c’est-\u00e0-dire les r\u00e9ponses cellulaires, la croissance\/diff\u00e9renciation cellulaire, la r\u00e9gulation du cycle cellulaire et la prolif\u00e9ration\/mort cellulaire. Aucune diff\u00e9rence significative n’a \u00e9t\u00e9 observ\u00e9e lors de la comparaison de l’expression des g\u00e8nes entre les deux groupes d’emplois (administratifs et sur site) ainsi qu’entre les deux groupes class\u00e9s sur la base des donn\u00e9es d’exposition aux MF.[\/vc_column_text][vc_custom_heading text=\u00a0\u00bbRapports d'activit\u00e9s pr\u00e9c\u00e9dents\u00a0\u00bb font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_custom_heading text=\u00a0\u00bb2017-2021″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_column_text]See\u00a0Cytogenetic biomonitoring of EMF exposed workers<\/a>[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb source=\u00a0\u00bb\u00a0\u00bb text=\u00a0\u00bb2013 – 2017″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb google_fonts=\u00a0\u00bbfont_family:Abril%20Fatface%3Aregular|font_style:400%20regular%3A400%3Anormal\u00a0\u00bb css_animation=\u00a0\u00bb\u00a0\u00bb link=\u00a0\u00bb\u00a0\u00bb el_id=\u00a0\u00bb\u00a0\u00bb el_class=\u00a0\u00bb\u00a0\u00bb css=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb][vc_custom_heading text=\u00a0\u00bbPublications\u00a0\u00bb font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_column_text]\n

Publications dans le cadre des activit\u00e9s du BBEMG<\/h3>\n

Nguyen H, Segers S, Ledent M, Anthonissen R, Verschaeve L, Hinsenkamp M, Collard JF, Feipel V, Mertens B.
Effects of long-term exposure to 50 Hz magnetic fields on cell viability, genetic damage, and sensitivity to mutagen-induced damage.<\/a>
Heliyon<\/strong>. 2023 Feb 27;9(3):e14097. doi: 10.1016\/j.heliyon.2023.e14097. eCollection 2023 Mar.
PMID: 36923833<\/p>\n

Nguyen H, Ledent M, Beauvois V, Anthonissen R, Verschaeve L, Collard JF, Hinsenkamp M, Feipel V, Mertens B.
In vitro 50 Hz magnetic field long-term exposure: Cytogenetic tests on human lymphoblastoid TK6 cells and validation of the test environment.<\/a>
MethodsX<\/strong>. 2020 Sep 26;7:101071. doi: 10.1016\/j.mex.2020.101071. eCollection 2020.
PMID: 33088726<\/p>\n

Collard JF, Hinsenkamp M.
Cellular processes involved in human epidermal cells exposed to extremely low frequency electric fields.<\/a>
Cell Signal.<\/strong> 2015 May;27(5):889-98. doi: 10.1016\/j.cellsig.2015.02.007. Epub 2015 Feb 12.
PMID: 25683910<\/p>\n

Collard J.-F., Lazar,C., Now\u00e9 A., & Hinsenkamp M.
Statistical validation of the acceleration of the differentiation at the expense of the proliferation in human epidermal cells exposed to extremely low frequency electric fields.<\/a>
Prog Biophys Mol Biol.<\/strong> 2013 Jan;111(1):37-45. doi: 10.1016\/j.pbiomolbio.2012.12.004. Epub 2012 Dec 19.
PMID: 23257322<\/p>\n

Hinsenkamp M.
Effets des champs \u00e9lectromagn\u00e9tiques sur la diff\u00e9renciation cellulaire et son int\u00e9r\u00eat en chirurgie orthop\u00e9dique et traumatologique.
Bulletin et M\u00e9moires de l’Acad\u00e9mie Royale de M\u00e9decine de Belgique<\/strong>. 166 (7-8-9):307-16, 2011.<\/p>\n

Hinsenkamp M, Collard JF.
Bone Morphogenic Protein–mRNA upregulation after exposure to low frequency electric field.<\/a>
Int Orthop<\/strong>. 2011 Oct;35(10):1577-81. doi: 10.1007\/s00264-011-1215-9. Epub 2011 Feb 10.
PMID: 21308375<\/p>\n

Collard, J.-F., Mertens, B. & Hinsenkamp, M.
In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells.<\/a>
Bioelectromagnetics<\/strong>, 2011 Jan;32(1):28-36. doi: 10.1002\/bem.20608.
PMID: 20809503[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=\u00a0\u00bbin_container\u00a0\u00bb full_screen_row_position=\u00a0\u00bbmiddle\u00a0\u00bb column_margin=\u00a0\u00bbdefault\u00a0\u00bb column_direction=\u00a0\u00bbdefault\u00a0\u00bb column_direction_tablet=\u00a0\u00bbdefault\u00a0\u00bb column_direction_phone=\u00a0\u00bbdefault\u00a0\u00bb scene_position=\u00a0\u00bbcenter\u00a0\u00bb text_color=\u00a0\u00bbdark\u00a0\u00bb text_align=\u00a0\u00bbleft\u00a0\u00bb row_border_radius=\u00a0\u00bbnone\u00a0\u00bb row_border_radius_applies=\u00a0\u00bbbg\u00a0\u00bb overflow=\u00a0\u00bbvisible\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb shape_divider_position=\u00a0\u00bbbottom\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb source=\u00a0\u00bb\u00a0\u00bb text=\u00a0\u00bb2013 – 2017″ font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb google_fonts=\u00a0\u00bbfont_family:Abril%20Fatface%3Aregular|font_style:400%20regular%3A400%3Anormal\u00a0\u00bb css_animation=\u00a0\u00bb\u00a0\u00bb link=\u00a0\u00bb\u00a0\u00bb el_id=\u00a0\u00bb\u00a0\u00bb el_class=\u00a0\u00bb\u00a0\u00bb css=\u00a0\u00bb\u00a0\u00bb][vc_column column_padding=\u00a0\u00bbno-extra-padding\u00a0\u00bb column_padding_tablet=\u00a0\u00bbinherit\u00a0\u00bb column_padding_phone=\u00a0\u00bbinherit\u00a0\u00bb column_padding_position=\u00a0\u00bball\u00a0\u00bb column_element_direction_desktop=\u00a0\u00bbdefault\u00a0\u00bb column_element_spacing=\u00a0\u00bbdefault\u00a0\u00bb desktop_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb tablet_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb phone_text_alignment=\u00a0\u00bbdefault\u00a0\u00bb background_color_opacity=\u00a0\u00bb1″ background_hover_color_opacity=\u00a0\u00bb1″ column_backdrop_filter=\u00a0\u00bbnone\u00a0\u00bb column_shadow=\u00a0\u00bbnone\u00a0\u00bb column_border_radius=\u00a0\u00bbnone\u00a0\u00bb column_link_target=\u00a0\u00bb_self\u00a0\u00bb column_position=\u00a0\u00bbdefault\u00a0\u00bb gradient_direction=\u00a0\u00bbleft_to_right\u00a0\u00bb overlay_strength=\u00a0\u00bb0.3″ width=\u00a0\u00bb1\/1″ tablet_width_inherit=\u00a0\u00bbdefault\u00a0\u00bb animation_type=\u00a0\u00bbdefault\u00a0\u00bb bg_image_animation=\u00a0\u00bbnone\u00a0\u00bb border_type=\u00a0\u00bbsimple\u00a0\u00bb column_border_width=\u00a0\u00bbnone\u00a0\u00bb column_border_style=\u00a0\u00bbsolid\u00a0\u00bb][vc_custom_heading text=\u00a0\u00bbInformations compl\u00e9mentaires\u00a0\u00bb font_container=\u00a0\u00bbtag:h2|text_align:left|color:%2349b0e5″ use_theme_fonts=\u00a0\u00bbyes\u00a0\u00bb][vc_column_text css=\u00a0\u00bb\u00a0\u00bb text_direction=\u00a0\u00bbdefault\u00a0\u00bb]\n